
Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 토끼 유래 anti-Goat IgG (H+L) 교차흡착 2차 항체로, WB, IHC, ELISA 등에 사용 가능. 고순도 정제 및 인체·쥐 단백질 교차흡착 처리. PBS 기반 동결건조 형태로 장기 안정성 우수. 연구용 시약으로 높은 감도와 다양한 검출 시스템 호환.
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Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2556529 |
| Target | IgG |
| Cross Adsorption | Against human and rat serum proteins prior to conjugation |
| Antibody Form | Whole Antibody |
Product Specific Information
- Formulation update: As of 31/Mar/2024, formulation changed from PBS with 0.02% thimerosal to PBS without thimerosal. Lots >2855945 do not contain thimerosal.
- Storage: Store at -20°C until opened.
- Reconstitution: Add 1 mL PBS (pH 7.2) to yield 1 mg/mL stock solution.
- Stability:
- Without stabilizer: stable ~3 months at 4°C.
- With 50% glycerol (v/v): aliquot and store at -20°C, stable up to 1 year.
- With preservative (e.g., 0.02% thimerosal): stable up to 1 year at 4°C.
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are used for detection, sorting, or purification of target molecules.
Secondary antibodies provide versatility across detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically produced by immunizing host animals with pooled immunoglobulins from the target species, followed by purification or modification (e.g., immunoaffinity chromatography, fragmentation, conjugation).
Available Conjugate Formats
- Alexa Fluor 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, Biotin-XX
- DyLight 488, 550, 594, 650, 680, 800
- FITC, TRITC
- Custom conjugation available upon request
Regulatory Information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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