Invitrogen™

LanthaScreen™ TR-FRET BACE1 Assay Kit

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer`s자세히 알아보기

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimers disease (AD). In some cases, early-onset familial AD can be attributed to aSwedish` mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that focus on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase.

Invitrogen`s LanthaScreen™ TR-FRET BACE1 assay provides sensitive high-throughput screening for potential inhibitors of beta-secretase. The kit uses a terbium (Tb)-labeled anti-biotin antibody and a fluorescein-labeled BACE1-biotin substrate in a homogeneous TR-FRET assay format (Figure 1).

Contents and Storage:

The LanthaScreen™ TR-FRET BACE1 Assay Kit contains BACE1 protein, fluorescently labeled BACE1 substrate, Tb-labeled anti-biotin antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

사양

어세이β-Secretase Assay

제품라인LanthaScreen™

기질 특성Peptide-Based Substrate

기질 유형Beta Secretase Substrate

용도(애플리케이션)TR-FRET

수량800 x 20 μL Assays

배송 조건Dry Ice

타겟Beta Secretase (BACE1)

Unit SizeEach