Thermo Fisher Scientific Mouse IgG Isotype Control

Applications

Tested Dilution

Immunohistochemistry (IHC)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Isotype Control

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532952

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody`s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.