Thermo Fisher Scientific HSC70 Polyclonal Antibody
HSC70 단백질을 인식하는 Thermo Fisher Scientific의 Goat Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 인간 및 생쥐 반응성. 0.5 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.03–0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (EKLQGKINDEDKQK) corresponding to internal amino acids of HSPA8 (aa 556–569) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11004266 |
Product Specific Information
This antibody is predicted to react with canine, mouse, and rat based on sequence homology.
Tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
The HSP70 family consists of highly conserved proteins induced by various biological stresses, including heat stress, across different organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73): Constitutively expressed 72 kDa protein involved in uncoating clathrin-coated vesicles
- GRP78 (BiP): Glucose-regulated 78 kDa protein localized in the endoplasmic reticulum
- HSP75 (p75): 75 kDa mitochondrial protein
HSC70 binds to clathrin light chain loops to promote uncoating and can interact with the cytoskeleton to facilitate rearrangements. It stimulates lysosomal degradation of intracellular proteins and modulates aggregation and folding of mitochondrial precursor proteins in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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