Invitrogen™

Dynabeads™ His-Tag Isolation and Pulldown

Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high자세히 알아보기

Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets. Their small but uniform bead size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule. They enable single-step isolation of tagged molecules in manual or automated workflows that helps ensure good recovery of the target. Dynabeads His-Tag Isolation and Pulldown beads can also be a great starting point for other advanced experimental approaches where solid magnetic support is needed.

Benefits of Dynabeads His-Tag and Pulldown beads include:
• Performance—high sensitivity and reproducibility securing consistent results
• Efficiency—fast binding kinetics and high binding capacity
• Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Automation-ready—automation protocols available with the KingFisher Purification instruments

Good signal-to-noise for numerous applications
Dynabeads His-Tag Isolation and Pulldown beads are an ideal tool for isolation of tagged recombinant proteins. They are also suited for other applications with high signal-to-noise requirements. Using the principles of immobilized metal affinity binding together with the inert surface of Dynabeads magnetic beads helps ensure a clean capture with extremely low carryover of unwanted proteins. Since the bead surface is loaded with cobalt ions, it is also possible to make a stable and lasting bond with the target molecule through oxidation if the downstream applications require it. The beads are added to the solution containing the target and mixed for 5-10 minutes. After washing, the target is eluted off and ready for downstream applications.

More than just a pulldown
In addition to being a great tool for protein pulldown and isolation, the Dynabeads His-Tag Isolation and Pulldown beads can serve as a platform for target immobilization. The bead carries cobalt Co2+ ions, so it is possible to cement the bond between the bead and the target just by using mildly oxidative conditions, such as 10 mM hydrogen peroxide for 30–60 minutes. This oxidizes cobalt to the Co3+ state, making the connection between the bead and the target permanent. It will not elute in the commonly used elution buffers for IMAC. This can later be used for applications requiring a strongly bound target, without the risk of reduced target yield.

Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.

*Commercial supply *
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads His-Tag Isolation and Pulldown beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
• See our immunoprecipitation selection table, data, and references
• Dynabeads magnetic beads selection guide
• See magnets for Dynabeads magnetic beads separations
• KingFisher automation protocols
• Watch a video about the KingFisher Flex instrument

사양

결합 특성1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads

농도40 mg/mL

물질Magnetic Polystyrene

직경(미터법)1 μm

용도(장비)KingFisher Sample Purification System, DynaMag magnets

고처리량 호환성Yes

배송 조건Ambient temperature

샘플 종류Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts

표면 기능성Cobalt-based immobilized metal affinity chromatography (IMAC)

제품라인Dynabeads™

수량2 mL

용도(애플리케이션)Isolation of His-tagged Recombinant Proteins

타겟His-tagged proteins

유형Magnetic Beads

최종 제품 유형His-Tagged Protein

유통 기한36 months from date of manufacture

리간드 유형Nitrilotriacetic acid (NTA)

충분40 Tests

형식Magnetic beads

색상Brown

인증/적합성ISO9001 and ISO13485

규제 상태For Research Use Only

Unit SizeEach