Thermo Fisher Scientific eIF4A1 Recombinant Rabbit Monoclonal Antibody (3T7U10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:7,000-1:28,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

Immunocytochemistry (ICC/IF)

1:100-1:1,800

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3T7U10

Immunogen

Recombinant protein of human eIF4A1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.079 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3093746

Target Information

eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.