Invitrogen™

Collibri™ Library Amplification Master Mix with Primer Mix

Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master자세히 알아보기

Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master mix includes Platinum SuperFi DNA Polymerase in combination with a proprietary reaction buffer containing all necessary components for efficient library amplification with minimal bias and superior accuracy.

Feature include:
• High fidelity—minimal error rates similar to PCR-free methods
• High efficiency—high yields from minute input amounts
• Minimal bias—uniform amplification regardless of GC content

This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

Platinum SuperFi DNA Polymerase has both 5to 3 polymerase and 3to 5 exonuclease (proofreading) activities, but lacks 5to 3 exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.

사양

샘플 종류DNA, RNA

Fidelity (Taq 대비)≥100

GC-Rich PCR 성능High

용도(애플리케이션)Sequencing

핫 스타트Built-In Hot Start

반응 수50

오버행Blunt

제품라인Collibri™

제품 유형Library Amplification Master Mix

수량50 reactions

반응 형식SuperMix or Master Mix

배송 조건Dry Ice

테스트 시간Standard

형태Liquid

Unit SizeEach