Thermo Fisher Scientific CD11b Monoclonal Antibody (CA16.3E10)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

Neat

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Goat, Mustelid, Mink, Pig

Published species

Bovine, Dog

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CA16.3E10

Immunogen

Affinity purified beta-2 integrins from splenic lysate

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538103

Product Specific Information

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.