Thermo Fisher Scientific CAD Polyclonal Antibody
Rabbit polyclonal antibody targeting human CAD protein. Validated for WB, IHC, and IP applications. Suitable for research use with recommended dilution ranges provided. Supplied as liquid form, 0.2 mg/mL concentration, stored at 4°C.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | – |
| Immunohistochemistry (IHC) | 1:100–1:500 | – |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1650 and 1700 of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
The de novo synthesis of pyrimidine nucleotides is required for mammalian cell proliferation.
This gene encodes a trifunctional protein associated with the enzymatic activities of the first three enzymes in the six-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase.
This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, indicating a direct link between MAPK activation and de novo pyrimidine biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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