Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, TRITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Conjugate Formats

• AP
• Biotin
• FITC
• HRP
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• NovaFluor Yellow 590, 610, 660
• PE
• Rhodamine
• WesternDot 625
• Custom conjugation available upon request

Product Specific Information

This goat anti-rat IgG polyclonal antibody reacts with rat IgG and is suitable for indirect immunofluorescent staining in flow cytometric analysis.
Applications reported include flow cytometric analysis and immunohistochemical staining.

This antibody has been tested by flow cytometric detection of rat primary antibodies and can be used at ≤1 µg per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (typically 10⁵–10⁸ cells/test).
It is recommended to titrate the antibody for optimal performance in each assay.

Target Information

Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of rat antibodies.
Secondary antibodies provide versatility and enhanced sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
These reagents are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.