Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, TRITC, eBioscience

Applications

Tested Dilution

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1 µg/test

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

TRITC TRITC TRITC

View additional formats

• [AP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Blue 510](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 510/host/Goat/)
• [NovaFluor Blue 555](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 555/host/Goat/)
• [NovaFluor Blue 585](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 585/host/Goat/)
• [NovaFluor Blue 610-70S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 610-70S/host/Goat/)
• [NovaFluor Blue 660-120S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 660-120S/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/PE/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

552/578 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469694

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Description: This goat anti-rat IgG polyclonal antibody reacts with rat IgG and is useful for indirect immunofluorescent staining for flow cytometric analysis.

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This polyclonal antibody has been tested by flow cytometric detection of rat primary antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.