Thermo Fisher Scientific Mouse NGF 7S Subunit Native Protein

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (IHC)

• Assay-dependent

ELISA

• Assay-dependent

Functional Assay

• Assay-dependent

Control

• Assay-dependent

Product Specifications

Product Specific Information

Reconstitution: Reconstitute murine natural NGF 7S to a stock concentration of 100 µg/mL with 1 mL of sterile culture medium containing 1–2% (w/v) BSA.
Aliquot into single-use portions in polypropylene vials and store at -20°C to avoid repeated freeze/thaw cycles.
Do not store in dilute solution. Dilute immediately before use.
To avoid loss of protein or activity, prepare dilute solutions in appropriate buffer or medium containing at least 0.1% (w/v) BSA or 1–10% (v/v) serum.
Carrier protein (BSA or serum) is needed to stabilize the NGF protein.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and nonneuronal cells.
Its actions result from binding to specific cell surface receptors present as both high and low affinity sites.
Only the high affinity receptor form is capable of signal transduction.
Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for both the human and rat NGF receptor genes reveal greater than 90% homology between the two species.
NGF exhibits nerve growth stimulating activity and regulates growth and differentiation of sympathetic and certain sensory neurons.
It also stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF gene expression is linked to allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.