Thermo Fisher Scientific H3K27me3 Polyclonal Antibody
H3K27me3 표적에 특이적인 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 등 다양한 종에 반응. Western blot, IHC, ChIP 등 다중 응용 가능. 고순도 항원 친화 크로마토그래피 정제 및 안정적 액상 형태로 제공.
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- PA531817
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Thermo Fisher Scientific H3K27me3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | 3 publications |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunohistochemistry – Free Floating (IHC (Free)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
| ChIP assay (ChIP) | - | 3 publications |
| Peptide Array (Array) | 1:2,000 | - |
| Dot blot (DB) | Assay-dependent | - |
| Immunomicroscopy (IM) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Bovine, Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 22–36 of Human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549290 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, K562, THP-1, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
H3K27me3 is an epigenetic mark where histone H3 is trimethylated at lysine residue 27. It is associated with gene silencing and found predominantly in heterochromatin regions and inactive gene promoters.
This modification opposes the activating mark H3K4me3 and is linked to developmental regulation.
EZH2 (PRC2 complex) acts as the methyltransferase (“writer”), while UTX and JMJD3 serve as demethylases (“erasers”).
Dysregulation of H3K27me3 is implicated in diseases such as cancer.
H3K27me3 antibodies are commonly used in Western blot and ChIP assays to study histone modification patterns.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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