Thermo Fisher Scientific EZ-Link Sulfo-NHS-Biotinylation Kit

Thermo Fisher Scientific EZ-Link™ Sulfo-NHS-Biotinylation Kit

The Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit contains reagents sufficient for 10 biotinylation reactions (e.g., 1–10 mg antibody per reaction).
The EZ-Link Sulfo-NHS-Biotin reagent included in the kit is a short-chain, water-soluble biotinylation reagent used for labeling antibodies, proteins, and other molecules that have primary amines.

Features of EZ-Link Sulfo-NHS-Biotin

• Protein labeling: Biotinylate antibodies to facilitate immobilization, purification, or detection using streptavidin resins or probes
• Cell surface labeling: Biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive: Reacts with primary amines (-NH₂), such as lysine side-chains or the amino-termini of polypeptides
• Soluble: Charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible: Forms permanent amide bonds; spacer arm cannot be cleaved
• Very short: Spacer arm (total length added to target) is 13.5 Å; consists of the native biotin valeric acid group only

Sulfo-NHS-Biotin is the shortest of three similar EZ-Link reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins, and other primary amine-containing macromolecules in solution.
Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane-impermeable reagents.
Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents allow optimization of labeling and detection experiments where steric hindrance of biotin binding is an important factor.
Sulfo-NHS-Biotin is offered in several package sizes and as complete protein labeling kits.

We manufacture biotin reagents to ensure the highest possible product integrity, consistency, and performance for research applications.

Technical Information

N-Hydroxysulfosuccinimide (NHS) esters of biotin are popular biotinylation reagents. NHS-activated biotins react efficiently with primary amino groups (-NH₂) in alkaline buffers to form stable amide bonds.
Proteins such as antibodies typically have several primary amines available for labeling, including lysine (K) residues and the N-terminus of each polypeptide.

Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability, and cleavability.
Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvents (e.g., DMSO or DMF).
Sulfo-NHS biotins and pegylated variants are directly water soluble but not membrane permeable.
Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.

Related Products

• EZ-Link™ Sulfo-NHS-Biotin
• EZ-Link™ Micro Sulfo-NHS-Biotinylation Kit

Specifications