Thermo Fisher Scientific NEFL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunohistochemistry (IHC)

1:4,000

-

Immunocytochemistry (ICC/IF)

1:2,000

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Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human NF-L protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077181

Product Specific Information

PA1-10000 was made against a preparation of full length human recombinant NF-L protein. It binds NF-L from a variety of species including human, rat and mouse.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.