Thermo Fisher Scientific Human AMPK alpha-1/beta-2/gamma-3, GST Tag, His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag, His-tag

Molecular weight

90.3-56.6-81.4 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This AMPK (A1/B2/G3) recombinant human protein (full length) was expressed in insect cells.

AMPK (A1/B2/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z`-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

Target Information

AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha1, beta2 and gamma3 subunits. While the purified AMPK (A1/B2/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z`-LYTE™ assay technology to identify novel kinase inhibitors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.