Thermo Fisher Scientific ZO-1 Monoclonal Antibody (ZO1-1A12)
ZO-1 단백질을 인식하는 mouse monoclonal antibody로, 다양한 종에서 반응성이 확인됨. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 액상 형태로 제공되며, PBS 완충액에 0.1% sodium azide 포함. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.2–2 µg/mL | 118 |
| Immunohistochemistry (IHC) | – | 196 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | 16 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 8 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500 | 22 |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL | 281 |
| Flow Cytometry (Flow) | – | 3 |
| ELISA | 0.1–1.0 µg/mL | – |
| Immunoprecipitation (IP) | – | 5 |
| in situ PLA (PLA) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 48 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rhesus monkey |
| Published Species | Amphibian, Bovine, Chicken, Dog, Fish, Goat, Guinea pig, Hamster, Human, Mammal, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Tag, Virus, Xenopus, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZO1-1A12 |
| Immunogen | Human recombinant ZO-1 fusion protein encompassing amino acids 334–634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533147 |
Available Conjugated Formats
Product Specific Information
Antibody 33-9100 has been successfully used in ELISA, Immunofluorescence, and Western blot analysis of ZO-1.
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junctions, linking the actin cytoskeleton to the junctional complex.
ZO-1 expression is reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, while ZO-1 is also found at cardiac myocyte junctions.
ZO-1 may play a role in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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