Thermo Fisher Scientific RNF216 Polyclonal Antibody

Applications

Western Blot (WB)

• Assay-dependent

Immunoprecipitation (IP)

• 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by adapter molecules such as TRIF, TIRAP, and MyD88.
Several regulatory mechanisms control TLR signal transduction, including inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR.
Another mechanism involves ubiquitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A (RING finger protein) binds to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing their ubiquitization.
Overexpression of TRIAD3A promotes degradation of TLR4 and TLR9, reducing ligand-specific activation, while depletion enhances TLR activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.