Thermo Fisher Scientific HER-2/c-erbB-2/neu/CD340 Recombinant Rabbit Monoclonal Antibody (ERBB2, 4376R)
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HER2/c-erbB-2/neu 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. HEK293 발현 시스템에서 생산되며, Protein A/G로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ERBB2, 4376R |
| Immunogen | Recombinant protein encoding the extracellular domain of human c-erbB2 (amino acids 1201–1255) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors.
Herceptin, used in treatment of metastatic HER2-positive cancer, is a monoclonal antibody targeting this kinase.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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