Thermo Fisher Scientific Cytokeratin Pan Monoclonal Antibody (B311.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:125

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B311.1

Immunogen

Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1958797

Product Specific Information

High temperature antigenic unmasking technique or enzymatic predigestion is recommended with this antibody when using paraffin-embedded tissue sections. Positive control of skin or colonic carcinoma suggested.

This antibody detects Cytokeratin 10, Cytokeratin 13, Cytokeratin 18, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6 and Cytokeratin 8.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.