Thermo Fisher Scientific Cytokeratin Pan Monoclonal Antibody (B311.1)
다양한 사이토케라틴 단백질을 인식하는 마우스 단클론 항체로, 인간, 마우스, 랫, 소 시료에 반응합니다. Western blot, IHC, ICC, IP 등 다양한 응용에 적합하며, 파라핀 절편에서는 항원 복원 처리가 권장됩니다. 연구용으로만 사용 가능합니다.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:125 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B311.1 |
| Immunogen | Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1958797 |
Product Specific Information
- Paraffin-embedded tissue sections require high-temperature antigen unmasking or enzymatic predigestion for optimal staining.
- Positive control: skin or colonic carcinoma tissue recommended.
- This antibody detects Cytokeratin 4, 5, 6, 8, 10, 13, and 18.
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight from 40 kDa to 68 kDa and have an isoelectric pH between 4.9 and 7.8.
Expression of cytokeratin subtypes varies by epithelial cell type, differentiation stage, growth environment, and disease state.
These expression patterns are useful in distinguishing epithelial malignancies and are valuable tools in tumor diagnosis, cancer research, cytopathology, and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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