Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 737, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats Available

Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Brilliant UV 395, Brilliant UV 496, Brilliant UV 563, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 605, eFluor 450, FITC, NovaFluor Blue/Red/Yellow/Violet/UV 시리즈 등 다양한 형광체 옵션 제공.

Product Specific Information

The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, mature T helper cells, and at low levels on monocytes. CD4 is a receptor for HIV; SK3 blocks HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 antibodies recognize distinct epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume).

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, optimized for cytometers with UV (355 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize polymer dye interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:
Samples can be fixed using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C. Compatible with short-term methanol fixation (≤1 hour).

Excitation/Emission:
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. Expressed primarily in T helper cells, monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and other tissues. CD4 aids in T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures.

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