
Thermo Fisher Scientific Rat anti-Mouse IgE Secondary Antibody, HRP
Thermo Fisher의 Rat anti-Mouse IgE HRP 결합 2차 항체로, ELISA 및 동결 절편 면역조직화학에 적합합니다. Mouse IgE 검출용으로 제작되었으며, 높은 특이성과 신호 증폭 효과를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | LO-ME-3 |
| Immunogen | Proprietary Immunogen |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_1075918 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody recognizes the mouse epsilon heavy chain of immunoglobulin (determined by immunodot). It is useful as a secondary antibody in immunoassays such as ELISA (providing good binding on plastic) and in immunohistochemistry on frozen sections.
Available formats include:
- Unconjugated (MON5051)
- FITC conjugated (MON5051F)
- HRP conjugated (MON5050P)
- Biotin conjugated (MON5051B)
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility by supporting multiple detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, or label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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