Applied Biosystems™

TaqMan™ SNP Genotyping Assay, mouse

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA자세히 알아보기

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP), and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

The predesigned TaqMan SNP Genotyping Mouse Assay collection consists of over 10,000 assays. For targets of interest that are not covered by the predesigned assay collection, Custom TaqMan SNP Genotyping Assays are available to meet your research needs.

Benefits:
• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Guaranteed—if you are not satisfied with the performance of a TaqMan Assay, we`ll replace it at no cost, or credit your account*

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

사양

3`프라이머 수정None

5`프라이머 수정None

설명VIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2

형태Frozen

형식Made to Order

용도(장비)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler*

* If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR
system in order to detect and record fluorescent signals.

그린 기능Sustainable packaging

내부 프로브 수정VIC™ (5), FAM (5), MGB (Minor Groove Binder) (3`)

라벨 또는 염료FAM, VIC

제품라인TaqMan™

정제 방법Solid Phase Extraction (SPE)

순도 또는 품질 등급RNase-Free, DNase-Free

수량L (2400 reactions), Made to Order

배송 조건Room Temperature

종Mouse

타겟Over 10,000 mouse assays are available

용도(애플리케이션)Genotyping

농도80X

반응 수2400 reactions

Unit SizeEach