Thermo Fisher Scientific Cytokeratin 5 (KRT5) Recombinant Rabbit Monoclonal Antibody (KRT5, 4245R)
Recombinant rabbit monoclonal antibody against human Cytokeratin 5 (KRT5). Validated for IHC (paraffin) applications. Produced in HEK293 cells and purified via Protein A/G. Supplied as liquid, 1 mg/mL in PBS without preservative. For research use only.
- 카탈로그번호
- 3852-RBM5-P1ABX
- 판매단위
- pk
카탈로그
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KRT5, 4245R |
| Immunogen | Recombinant fragment (aa 490–590) corresponding to the C-terminal of human cytokeratin 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For IHC using PFA-fixed tissue: incubate antibody for 30 minutes at room temperature.
Formalin-fixed tissues require heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins with high biochemical diversity, represented by at least 20 different human epithelial polypeptides.
Cytokeratins range from 40–68 kDa with isoelectric points between 4.9–7.8.
Each epithelial tissue expresses characteristic cytokeratin patterns that reflect both tissue type and differentiation state.
Cytokeratin expression profiles are valuable for distinguishing epithelial malignancies and are widely used in tumor diagnosis, cytopathology, and flow cytometry.
Keratins serve as key markers in cancer research and tumor classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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