Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), Super Bright 645, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β TCR-bearing T cells, subsets of γ/δ T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein structurally similar to CD4. A soluble form of human CD223 has been detected, and mouse CD223 can be proteolytically cleaved into a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa form may remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and homeostatic proliferation. It is also expressed by CD4⁺CD25⁺ regulatory T cells, suggesting a role in immune regulation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes; ≤0.5 µg/test recommended (100 µL volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Recommended filter: 660/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal loss of signal.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

LAG-3 (Lymphocyte Activation Gene-3) is a 70 kDa surface glycoprotein in the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and negatively regulates T-cell activation and proliferation.
LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells. CD8⁺ T cells typically express higher levels of LAG-3 than CD4⁺ T cells. Coexpression of LAG-3 and CD49b marks Type 1 regulatory T cells (Tr1 cells) in humans and mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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