Thermo Fisher Scientific ICAM1 Monoclonal Antibody (OTI2H4), TrueMAB
인간 ICAM1 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. TrueMAB 포맷으로 높은 특이성과 재현성을 제공. 액상 형태, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 | - |
| Immunocytochemistry (ICC/IF) | 1:150 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H4 |
| Immunogen | Full length human recombinant protein of human ICAM1 produced in HEK293T cell. Target: ICAM-1 (CD54), UniProt ID: P05362-1, Antigen Range: 1–532 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain.
It has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
ICAM-1 binds to integrins such as CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1) and serves as a receptor for Rhinovirus.
It is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils, with expression increasing upon inflammation.
Soluble ICAM-1 is detectable in plasma and elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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