Thermo Fisher Scientific Rhesus IL-18 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Mature rhesus macaque IL-18 was expressed in Pichia pastoris X-33 (GenBank Accession # AAK13416, amino acids 37–193).
The expressed protein was N-terminal sequenced and begins with the following 10 amino acids: YFGKLESKLS.
Purity is >95% by SDS-PAGE analysis.

Activity:
Recombinant rhesus macaque IL-18 induces IFN-γ production from KG-1 cells in the presence of recombinant mouse IL-12.
270 pg/mL and 470 pg/mL of IFN-γ are induced by 20 ng/mL and 40 ng/mL of recombinant rhesus macaque IL-18, respectively.

Use:
For in vitro applications. Perform dose-response assays to determine optimal concentration for specific experiments.

Reconstitution and Dilution:

• Store lyophilized material at 4°C.
• Reconstitute in 0.5 mL distilled or deionized water.
• Dilute in buffer containing carrier protein, aliquot, and store at or below –70°C.
• Avoid multiple freeze-thaw cycles.
• Suitable buffers: PBS with 1% BSA or neutral media containing <10% FCS.

Target Information

The protein encoded by this gene is a proinflammatory cytokine that induces IFN-γ production in T cells.
In combination with IL-12, it inhibits IL-4–dependent IgE and IgG1 production, while enhancing IgG2a production in B cells.
IL-18 binding protein can specifically interact with this cytokine to negatively regulate its biological activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.