Thermo Scientific™

SfiI (10 U/μL)

5G G C C N N N N ↓N G G C C 3 3` C C G G자세히 알아보기

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5`			G	G	C	C	N	N	N	N ↓	N	G	G	C	C		3`
3`			C	C	G	G	N ↑	N	N	N	N	C	C	G	G		5`

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Thermo Scientific SfiI restriction enzyme recognizes GGCCNNNN^NGGCC sites and cuts best at 50°C in G buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Assayed using pUC19 DNA with insert containing two SfiI sites from Adenovirus-2 DNA. Incubation at 37°C results in 10% activity. SfiI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163. For cleavage with SfiI, at least two copies of its recognition sequence are required. The two sites can be on either the same or different DNA molecules. (L. M. Wertzell et al., J. Mol. Biol., 248, 581-595, 1995.) Therefore, also an oligonucleotide harboring a SfiI recognition site can be supplemented. For methylation sensitivity, refer to product specifications.

사양

농도10 U/μL

메틸화 민감도CpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive

효소SfiI

함께 사용가능한 버퍼10X Buffer G

열 불활성화에 대한 민감성No

최적 반응 온도50°C

Type IIS RENo

수량1,000 units

제품 유형Restriction Enzyme

연구 카테고리Traditional Cloning

Unit SizeEach