Thermo Fisher Scientific IkB epsilon Monoclonal Antibody (OTI4D1)
Human IkB epsilon 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot에 적합. 비결합(Unconjugated) 형태이며 1 mg/mL 농도의 액상 제품. PBS/BSA/glycerol 버퍼에 보관하며 -20°C에서 안정적. NF-κB 신호전달 연구용으로 최적화됨.
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Thermo Fisher Scientific IkB epsilon Monoclonal Antibody (OTI4D1)
Applications
- Western Blot (WB): 1:500–1:2,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D1 |
| Immunogen | Full-length human recombinant protein of NFKBIE produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724509 |
Target Information
NFKB1 or NFKB2 binds to REL, RELA, or RELB to form the NF-κB complex. This complex is inhibited by IκB proteins (NFKBIA or NFKBIB), which trap NF-κB in the cytoplasm. Phosphorylation of serine residues by kinases (IKBKA or IKBKB) targets IκB proteins for ubiquitination and degradation, thereby activating NF-κB. The activated complex translocates to the nucleus and binds DNA at κB-binding motifs such as 5'-GGGRNNYYCC-3' or 5'-HGGARNYYCC-3'. NF-κB activation may require interactions with other transcription factors including STAT6, AP1 (JUN), and NFAT (NFATC1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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