Thermo Fisher Scientific Myoglobin Recombinant Rabbit Monoclonal Antibody (ZR69), RAbMono

Thermo Fisher Scientific Myoglobin Recombinant Rabbit Monoclonal Antibody (ZR69), RAbMono

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) (IHC (P)): 1:100–1:200

Product Specifications

Product Specific Information

A recommended positive control tissue for this product is skeletal muscle, though other tissues may also be used.

This antibody is intended for laboratory professional use in detecting Myoglobin in formalin-fixed, paraffin-embedded tissues using manual qualitative immunohistochemistry (IHC). It is used after the primary diagnosis of tumors by conventional histopathology.

Myoglobin Function and Diagnostic Use:

• Myoglobin is a cytosolic oxygen-binding protein responsible for oxygen storage and diffusion in myocytes.
• Expression is highest in skeletal and cardiac muscle.
• Acts as an O₂-storage protein, releasing oxygen during hypoxia or anoxia.
• Useful in identifying rhabdomyosarcoma and tumors with skeletal muscle differentiation.
• Reportedly expressed in epithelial cancer cells under altered metabolic conditions.

Staining and Controls:

• Use with formalin-fixed and paraffin-embedded sections.
• Pretreatment with heat-induced epitope retrieval or enzymatic retrieval recommended.
• IHC staining involves sequential application of primary antibody, secondary antibody, enzyme complex, and chromogenic substrate.
• A positive tissue control must be run with every staining procedure.
• Positive control tissues should contain both positive and negative staining components.
• External positive controls should be properly fixed and embedded specimens.
• Weakly positive tissues are recommended to detect subtle changes in antibody sensitivity.
• Internal or external negative controls may be used as per institutional guidelines.

Target Information

This gene encodes a member of the globin superfamily, expressed in skeletal and cardiac muscle. The protein is a hemoprotein that contributes to intracellular oxygen storage and transcellular oxygen diffusion.
At least three alternatively spliced transcript variants encoding the same protein have been reported.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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