Thermo Fisher Scientific ISG15 Recombinant Rabbit Monoclonal Antibody (HL2017)

Applications and Tested Dilution

Product Specifications

Target Information

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MHC-restricted cytotoxicity.

Synthesis is stimulated by IFN-alpha, IFN-beta, or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors involved in transcriptional regulation of IFN genes, and by influenza B virus. It is secreted by monocyte, T-lymphocyte, B-lymphocyte, human fibroblast, and epithelial cell lines.

Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade, suggesting its role in amplifying and directing immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 also functions intracellularly as a ubiquitin homolog. Mutations in the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.