Thermo Fisher Scientific CD11b Recombinant Mouse Monoclonal Antibody (107)

Applications

Flow Cytometry (Flow)

• Tested Dilution: Assay-dependent

ELISA (ELISA)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Specificity: This antibody binds to the MIDAS face of the A domain from integrin CD11b.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).

CD11b is expressed on the surface of:

• Monocytes/macrophages
• Granulocytes
• Activated lymphocytes
• A subset of NK cells
• A subset of dendritic cells
• Microglia in the brain

CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These adhesions are critical for cell-cell and cell-matrix interactions.

Expression details:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

Commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies among tissue macrophages. Peritoneal macrophages express higher levels of CD11b than splenic macrophages.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.