Thermo Fisher Scientific His tag Polyclonal Antibody
His tag 검출용 Rabbit Polyclonal Antibody로 다양한 종에서 사용 가능. Western blot 및 IHC에 적합하며, 합성 hexa-His 항원으로 제작됨. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적 유지 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific His tag Polyclonal Antibody
Applications
Western Blot (WB)
- Tested Dilution: 10–50 µg/mL
Immunohistochemistry (IHC)
- Tested Dilution: 10–50 µg/mL
Miscellaneous (PubMed)
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic hexa His (HHHHHH) conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: His tag (HHHHHH).
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins even when specific antibodies are unavailable.
His-tagged proteins are purified via metal chelate chromatography due to the strong affinity between histidine clusters and metal ions such as Ni²⁺.
His tag-specific antibodies are useful for detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.