Thermo Fisher Scientific Human PPARA, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Ligand Binding Assay (LBA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

192-468

Tag

GST-tag

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

proprietary buffer

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

PPARA (peroxisome proliferator activated receptor alpha) ligand binding domain is a 59.40 kDa recombinant human protein (amino acids 192-468) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. PPAR alpha-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Protein Form: Ligand Binding Domain, Recombinant

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR`s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.