Thermo Fisher Scientific Bcl-X Polyclonal Antibody
Bcl-X 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며 고순도의 항원 친화 크로마토그래피로 정제되었습니다. 세포 사멸 연구 및 미토콘드리아 기능 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–208 of Bcl-X (Uniprot ID: Q07817) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1 M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154645 |
Product Specific Information
- Recommended positive controls: HCT116, NIH-3T3, PC-12, OKT3
- Predicted reactivity: Mouse (97%), Rat (97%), Dog (100%), Cat (99%), Pig (97%), Rabbit (96%), Sheep (98%), Rhesus Monkey (99%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by the BCL-X gene belongs to the BCL-2 protein family. Members of this family form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities. These proteins are located at the outer mitochondrial membrane and regulate the mitochondrial membrane channel (VDAC) opening. VDAC controls mitochondrial membrane potential, reactive oxygen species production, and cytochrome C release—key events in apoptosis induction.
Alternative splicing results in multiple transcript variants encoding two isoforms: the longer isoform acts as an apoptotic inhibitor, and the shorter isoform acts as an apoptotic activator.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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