Thermo Fisher Scientific Carbonic Anhydrase VB Polyclonal Antibody
Thermo Fisher Scientific의 Carbonic Anhydrase VB Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 CA VB 단백질 검출용으로 개발된 토끼 IgG 항체입니다. Western blot, ICC/IF, ELISA에 사용 가능하며, 고순도 친화 크로마토그래피로 정제되었습니다. 미토콘드리아 내 CA VB 연구에 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–317 of human CA5B (NP_009151.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914139 |
Product Specific Information
Positive test controls include: A-549, Mouse kidney, Mouse lung, Mouse spleen, Rat lung.
The target is usually found in the following locations: Mitochondrion.
Immunogen sequence:
MVVMNSLRVI LQASPGKLLW RKFQIPRFMP ARPCSLYTCT YKTRNRALHP LWESVDLVPG GDRQSPINIR WRDSVYDPGL KPLTISYDPA TCLHVWNNGY SFLVEFEDST DKSVIKGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCF PAELHLVHWN AVRFENFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDALV EFGSFDPSCL MPTCPDYWTY SGSLTTPPLS ESVTWIIKKQ PVEVDHDQLE QFRTLLFTSE GEKEKRMVDN FRPLQPLMNR TVRSSFRHDY VLNVQAKPKP ATSQATP
Target Information
Carbonic anhydrases (CAs) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.
They exhibit extensive diversity in distribution and subcellular localization and participate in various biological processes such as calcification, bone resorption, respiration, acid-base balance, and formation of aqueous humor, saliva, gastric juice, and cerebrospinal fluid.
CA VB (Carbonate Dehydratase VB) is one of two isoforms of CA V, localized to mitochondria and involved in metabolic processes.
It is predominantly expressed in heart, pancreas, lung, placenta, kidney, and skeletal muscle.
Unlike CA VA, CA VB is not expressed in the liver, suggesting a distinct physiological role.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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