Thermo Fisher Scientific Horse anti-Mouse IgG (H+L) Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

Assay-dependent

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Horse / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 20mg/mL sucrose

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient

RRID

AB_228364

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 31181 has been successfully used in Western blot, IF, ICC, IHC, ELISA and ISH applications.

Product # 31181 reacts with heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rat IgG and mouse IgM (25%), hamster IgG (12%), guinea pig IgG and rat IgM (6%), bovine IgM, dog IgM, goat IgM, human IgM, Rhesus monkey IgG, sheep IgG, sheep IgM, swine IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species.

The activity measured by a solid phase binding assay is 94%.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.