Thermo Fisher Scientific TRIzol Max Bacterial RNA Isolation Kit, 100 Preps

Invitrogen™ TRIzol™ Max™ Bacterial RNA Isolation Kit

The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol™ Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features

• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit

The TRIzol™ Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol™ Reagent.
A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent, and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases.
Subsequent bacterial lysis by TRIzol™ Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA Applications

Bacterial RNA isolated using this kit is suitable for downstream applications such as:

• Real-time PCR (qPCR)
• RT-PCR
• Northern blotting
• Nuclease protection assays
• cDNA synthesis
• Microarray analysis
• Dot blot hybridization

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

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