Thermo Fisher Scientific CD3 Monoclonal Antibody (KT3), FITC

Applications

• Flow Cytometry (Flow)
  Tested Dilution: Neat-1:5
  View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• PE
• Request custom conjugation

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Rat anti-Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, which is expressed by mature T cells.

Target Information

The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex.
T cell activation through the TCR involves the cytoplasmic tails of the CD3 subunits (gamma, delta, epsilon, zeta), which are structurally related members of the immunoglobulin superfamily encoded by genes on human chromosome 11.
CD3 components associate with cytoplasmic signal transduction molecules via a double tyrosine-based motif.
The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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