Thermo Fisher Scientific SAE1 Polyclonal Antibody
Human SAE1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 고순도 Protein A 정제 및 Lyophilized 형태로 제공. 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human SUMO Activating Enzyme E1 (baculoviral expression in Sf9 cells). |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This purified antibody is directed against human SUMO Activating Enzyme E1 protein.
BLAST analysis suggests reactivity with SUMO Activating Enzyme E1 proteins from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on sequence homology.
Cross-reactivity with other species has not been determined.
Target Information
Posttranslational modification of proteins by SUMO (Small Ubiquitin-like Modifier) regulates protein structure and intracellular localization.
SAE1 and UBA2 form a heterodimer functioning as a SUMO-activating enzyme for protein sumoylation (Okuma et al., 1999).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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