Thermo Fisher Scientific PRPF40A Polyclonal Antibody
Human PRPF40A 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며 항원 친화 크로마토그래피로 정제됨. 액상 형태, 0.3 mg/mL 농도, 장기 보관 시 -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PRPF40A. Recombinant protein control fragment (Product #RP-102820). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645974 |
Product Specific Information
Immunogen sequence:
SQTKESRWAK PKELEDLEGY QNTIVAGSLI TKSNLHAMIK AEESSKQEEC TTTSTAPVPT TEI
Highest antigen sequence identity to the following orthologs:
- Mouse: 97%
- Rat: 98%
Target Information
FNBP3 (Formin-binding protein 3) is a 957 amino acid protein encoded by the human gene PRPF40A.
It belongs to the PRPF40 family and contains five FF domains and two WW domains. Nuclear proteins with both WW and FF interaction modules bind to splicing factors and RNA polymerase II, linking transcription with splicing.
Through the WW domains, FNBP3 binds to Formin proline-rich regions. Also known as Pre-mRNA-processing factor 40 homolog A, FNBP3 interacts with the WASL/N-WASP complex and suppresses its translocation from the nucleus to the cytoplasm, inhibiting cytoplasmic function.
FNBP3 is widely expressed in most tissues and localized to nuclear speckles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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