Thermo Fisher Scientific Laminin gamma-2 Polyclonal Antibody
LAMC2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 면역세포염색(ICC/IF)에 적합. 인간 시료 반응성. Affinity chromatography로 정제되었으며, PBS/glycerol buffer에 보관. 세포 부착 및 기저막 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LAMC2 (Accession Q13753), corresponding to amino acid residues P426–T476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852822 |
Product Specific Information
Antibody detects endogenous levels of total LAMC2.
Target Information
Laminins are extracellular matrix glycoproteins and major noncollagenous components of basement membranes. They are involved in cell adhesion, differentiation, migration, signaling, neurite outgrowth, and metastasis. Laminins consist of three chains—alpha, beta, and gamma—forming a cruciform structure with three short arms and one long arm.
The Laminin gamma-2 (LAMC2) chain is highly homologous to the gamma-1 chain but lacks domain VI and has shorter domains V, IV, and III. It is expressed in epithelial cells of skin, lung, and kidney, forming part of Laminin-5 (Kalinin) with alpha-3 and beta-3 chains, which anchor epithelial cells to the basement membrane. Mutations in LAMC2 are associated with junctional epidermolysis bullosa, a blistering skin disorder. Two transcript variants encoding different isoforms have been described, showing differential expression in embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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