Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (MGL3R), APC
Ubiquitin 단백질 검출용 APC-conjugated mouse monoclonal antibody. Western blot 및 ELISA에 적합하며, poly-ubiquitinated 단백질(K29, K48, K63 연결형)을 특이적으로 인식. 단백질 분해 연구 및 단백질 변형 분석에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MGL3R |
| Immunogen | Ubiquitin conjugated lysozyme |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 47.35 mM phosphate / 2.15 mM MES, pH 7.4, with 1.033 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931532 |
Additional Formats
- FITC (Cat. No. MA5-45079)
- PerCP (Cat. No. MA5-45080)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45078 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Ub1–Ub7 WT Chains, K63-linked, K48-linked) by ECL immunoblot using goat anti-mouse IgG:HRP as the secondary antibody.
Detects ubiquitinated proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor consisting of either polyubiquitin chains or a single ubiquitin fused to another protein.
This gene encodes a fusion protein with ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. The expressed protein is post-translationally processed to generate free ubiquitin and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is localized in the cytoplasm. Pseudogenes derived from this gene exist in the genome. Similar fusion proteins include ribosomal proteins L40 and S30, which are synthesized with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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