Thermo Fisher Scientific CTCF Polyclonal Antibody
CTCF 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, ICC, IP, ChIP, RIP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 등 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로, -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:800 | - |
| Immunoprecipitation (IP) | 1:50 | - |
| ChIP assay (ChIP) | 10 µl/10^6 cells | - |
| RNA Immunoprecipitation (RIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of the human CTCF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 14 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10982283 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with BORIS.
Target Information
CTCF (CCCTC-binding factor) is a ubiquitously expressed nuclear phosphoprotein with 11 zinc finger domains. It plays multiple roles in gene regulation, including transcriptional repression and activation, gene silencing, chromatin insulation, and regulation of imprinted sites. CTCF interacts with DNA at the CCCTC motif and can recruit HAT- and HDAC-containing complexes to modulate transcriptional activity.
It is involved in the regulation of genes such as APPB, c-myc, Pax6, and IRAK2, contributing to chromatin organization and genomic imprinting (e.g., IGF2 and H19 loci). Mutations in CTCF have been associated with various cancers, including breast, prostate, and Wilms’ tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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