Thermo Fisher Scientific TLR5 Monoclonal Antibody (85B152.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Flow Cytometry (Flow)

1-3 µg per million cells in 100 µl

-

Product Specifications

Species Reactivity

Dog, Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

85B152.5

Immunogen

KLH-conjugated synthetic peptide corresponding to a portion of human TLR5.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537751

Product Specific Information

Suggested positive control: Ramos.

Target Information

TLR5 is a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. TLR5 also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. TLR5 protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has a unique TLR5 haplotype and a lower expression of TRL5. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires` disease and demonstrates the importance of TLR5 in the innate immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.