Thermo Fisher Scientific TET3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry - Free Floating (IHC (Free))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Immunoprecipitation (IP)

1:500-1:1,000

-

ChIP assay (ChIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Xenopus

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1456 and 1660 of Human TET3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TET3,uniProtId:O43151-1,ncbiNodeId:9606,antigenRange:1456-1660,antigenLength:1795,antigenImageFileName:PA5-31860_TET3_O43151-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31860_TET3_O43151-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549333

Product Specific Information

Recommended positive controls: Flag-human TET3 transfected 293T.

Predicted reactivity: Mouse (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.