Thermo Fisher Scientific TET3 Polyclonal Antibody
TET3 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC, IP, ChIP 등 다양한 응용 가능. 고순도 항원 친화 크로마토그래피로 정제. 인간, 생쥐 등 여러 종에 반응하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunohistochemistry - Free Floating (IHC-Free) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:500–1:1,000 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Xenopus |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1456–1660 of Human TET3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.14 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2549333 |
Product Specific Information
- Recommended positive control: Flag-human TET3 transfected 293T cells
- Predicted reactivity: Mouse (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TET3 is a member of the ten-eleven-translocation (TET) family of genes and acts as a methylcytosine dioxygenase that converts methylcytosine to 5-hydroxymethylcytosine. It is highly expressed in hematopoietic cells. Unlike TET2, mutations in TET3 have not been observed in myeloid malignancies. TET3 is involved in demethylation of zygotic DNA before the first mitosis and plays a role in epigenetic reprogramming of paternal DNA after fertilization, as well as in somatic cell nuclear reprogramming during cloning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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