Thermo Fisher Scientific PHGDH Polyclonal Antibody
PHGDH 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, IP에 적합합니다. Human, Mouse, Rat 반응성이 있으며, 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 264–533 of human PHGDH (NP_0066142) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.33 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804504 |
Product Specific Information
Immunogen sequence:
EEPPRDRALV DHENVISCPH LGASTKEAQS RCGEEIAVQF VDMVKGKSLT GVVNAQALTS AFSPHTKPWI GLAEALGTLM RAWAGSPKGT IQVITQGTSL KNAGNCLSPA VIVGLLKEAS KQADVNLVNA KLLVKEAGLN VTTSHSPAAP GEQGFGECLL AVALAGAPYQ AVGLVQGTTP VLQGLNGAVF RPEVPLRRDL PLLLFRTQTS DPAMLPTMIG LLAEAGVRLL SYQTSLVSDG ETWHVMGISS LLPSLEAWKQ HVTEAFQFHF
Positive Samples:
U-87MG, B cells, HeLa, SKOV3, Mouse brain, Rat spinal cord
Target Information
The PHGDH gene encodes an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is essential for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been linked to congenital microcephaly, psychomotor retardation, and related symptoms. Multiple alternatively spliced transcript variants exist, though the full-length nature of most is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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