Thermo Fisher Scientific Dog MRP2 Vesicles

Gibco™ Dog MRP2 Vesicles

Dog MRP2 Vesicles are insect-derived purified plasma membranes with inserted MRP2 transport protein (multidrug resistance-associated protein, ABCC2). These vesicles can be used to evaluate test compounds and drug interactions with MRP2 in in vitro assays. MRP2 is an ATP-binding cassette (ABC) transporter that carries organic anions.

주요 용도

• Investigate transporter interactions of drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high-quality results with large signal-to-noise ratios

ABC Transporter Vesicle 개념

ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate’s substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for a compound by determining the amount incorporated into the vesicles.

명확하고 신뢰할 수 있는 결과

Prepared from Sf9 cells engineered to over-express specific ABC transporters, these “inside-out” vesicles provide high levels of transporter activity with low background, giving a clear signal if a compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle 세부 정보

Dog MRP2 Vesicles are part of the ABC transporter vesicle group. They are prepared from purified plasma membranes isolated from insect cell systems (Sf9 cells transfected with baculovirus) expressing MRP2. MRP2 is expressed in various organs and plays a role in excretion of compounds from the kidney and liver, based on its localization on the canalicular plasma membrane of hepatocytes and brush border membrane of tubular epithelial cells.

제품 사양