Thermo Fisher Scientific COX2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit.
This replacement antibody is recommended for researchers needing an antibody for high content cell-based assays.
It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications.
Further optimization, including the selection of the most appropriate fluorescent Dylight-conjugated secondary antibody, may be required for your high-content assay.

It is not recommended to aliquot this antibody.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins.
This conversion involves two reactions:

1. Cyclooxygenase (COX) reaction: arachidonate → prostaglandin G2 (PGG2)
2. Peroxidase reaction: PGG2 → prostaglandin H2 (PGH2)

COX2 is constitutively expressed in some tissues (endothelium, kidney, brain) and up-regulated under pathological conditions such as cancer and inflammation.
Up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 is a key step in producing prostaglandin E2 (PGE2), which modulates motility, proliferation, and apoptosis resistance.
COX2 is naturally inhibited by calcitriol (active Vitamin D).
Glucocorticoids repress PTGS2 gene activity in vivo, partly by interfering with transcription initiation and elongation.
COX2 is a known target of NSAIDs such as aspirin, which reduce pain and inflammation driven by COX2 activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.