
Thermo Fisher Scientific SF1 Monoclonal Antibody (OTI4C9), TrueMAB
Human SF1 단백질(aa 1-276)을 면역원으로 한 Mouse monoclonal antibody. Western blot에 1:500–1:2000 희석으로 사용 가능. Affinity chromatography로 정제된 액상형 항체로, PBS/BSA/glycerol buffer에 보관. -20°C에서 보관하며 동결·해동 반복은 피해야 함.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–276 of human SF1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF). It is essential for early stages of spliceosome assembly and also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region (binds branch-site adenosine)
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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