Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain. This receptor is expressed on subsets of γδ T cells, NK (CD56^dim^), CD8⁺, and CD4⁺ T cells. KLRG1 is mainly found on effector/memory phenotype cells that are short-lived but capable of immediate effector function.

Cadherin-E, -N, and -R are ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation. The proportion of KLRG1⁺ cells varies depending on antigen exposure. Clones 13F12F2 and 13A2 recognize a similar epitope based on cross-blocking studies.

• Tested by flow cytometric analysis of human peripheral blood cells
• Recommended usage: ≤0.5 µg per test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Filtration: 0.2 µm post-manufacturing filtered
• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric receptor of the lectin-like type 2 transmembrane family. It contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that interact with SH2 domains of phosphatases such as SHP-1. KLRG1 acts as an inhibitory receptor expressed on NK cells and certain T cells, playing a role in regulating immune responses and cytolytic activity against tumor and virus-infected cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.